I will cut right to the point: above all, have an IT Security Policy in place. Data loss and damage to reputation from ignorance rather than deliberate sabotage is easier to prevent and far more likely - let your staff or co-workers know what is and is not acceptable. Your biggest and most likely risks are loss or theft of data, including damage through viruses and worms - not hackers.
Assuming all the basics are in place (backup, anti-virus, etc.), having a policy in place which contains some basic procedures is pretty much the most important thing you can do. This will allow you to control the flow of data into and out of your organisation and better protect it.
Policies will have to be different for each organisation, but I want to give a few ideas you could consider:
Security of backup media: You may well be in the habit of taking tapes off-site, despite widely publicized security lapses in government and large organisations, a significant proportion of backup tapes are being ported around without even basic security. Check if data on the tapes is encrypted - many backup systems do not do this by default. If your backup tapes cannot be encrypted, consider whether they should be in a pocket or handbag on trains, buses etc.
Database Security: Can people take your data easily. Examine the risks, it is commonplace for data to be transferred straight out of networks via FTP, Google Documents or even a Hotmail account. These holes can be closed.
USB Drives: In a survey last month, almost a third of those questioned admitted that they had lost a USB key. Of these, only a really small proportion had encrypted the contents. Is it worthwhile telling staff not to use portable drives?
External email: Encouraging personal use of business email may sound risky, but weigh it up against the risks of staff using less protected email systems that could bypass security and scanning you already have in place and be an easy way for viruses to inadvertently get into your network.
Mobile devices: If you allow staff to collect, send and store business email on mobile devices (Blackberries, iPhones, etc.) the risk of data loss or theft is significant - Last weekend the story broke of the Blackberry purchased on ebay containing contact details of dozens of A-List celebrities - according to press reports it was in fact lost over 18 months ago - it could in fact have been remotely wiped in minutes if it was set up as part of a business system.
Passwords - give people ideas on how to create a secure password, tell people they shouldn't give them out to co-workers.